Alterations in plasma membrane of glioblastoma cells by photodynamic action of merocyanine 540
نویسندگان
چکیده
منابع مشابه
Alterations in plasma membrane of glioblastoma cells by photodynamic action of merocyanine 540.
Photodynamic action of merocyanine 540 (MC540) on the plasma membrane of human glioblastoma(U-87MG) cells has been investigated. Plasma membrane was labeled with lipid specific probe 1,(4-trimethylammonium),6-diphenyl-1,3,5-hexatriene. Steady-state anisotropy, decay time and time-dependent anisotropy of TMA-DPH in U-87MG cells have been measured as a function of light dose. A decrease in the st...
متن کاملPhotodynamic action of merocyanine 540 on carcinoma of cervix cells.
Results of the studies carried out on localization and photodynamic action of merocyanine 540 (MC540) on carcinoma of cervix (HeLa) cells are presented. Fluorescence microscopic study showed that when HeLa cells were incubated with MC540 in dark, the dye localized in plasma membrane of cells. Photoirradiation of cells in presence of MC540 led to enhancement of dye uptake, intracellular localiza...
متن کاملPhotodynamic action of merocyanine 540 on artificial and natural cell membranes: involvement of singlet molecular oxygen.
The photochemistry of merocyanine 540 (MC 540), a sensitizing dye that binds preferentially to leukemia and electrically excitable cells, has been investigated. MC 540-mediated photooxidation of histidine, arachidonate, and unsaturated phospholipid vesicles was assessed by spin label oximetry and shown to involve type II (singlet oxygen) chemistry. The dye was also shown to be a potent sensitiz...
متن کاملUse of merocyanine 540 for photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells.
Photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells by a phtotosensitizer, merocyanine 540 (MC 540), was investigated. For the planktonic experiments, MC 540 binding efficiency to bacterial cells was found to increase with both increasing MC 540 concentration and increasing incubation time, but the binding became saturated following 10 min of incubation. The antimicr...
متن کاملMerocyanine 540 as a flow cytometric probe of membrane lipid organization in leukocytes.
Merocyanine 540 (MC540) is a fluorescent probe that binds preferentially to membranes with loosely packed lipids. When combined with flow cytometry, it provides a novel methodology for rapidly and quantitatively assessing lipid organization in individual leukocytes. Analysis of cells stained simultaneously with MC540 and 1-[4-trimethylammoniumphenyl]-6-phenyl-1,3,5-hexatriene, to normalize for ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1997
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(96)00195-2